Improved quantification method of crocins in saffron extract using HPLC-DAD after qualification by HPLC-DAD-MS

Crocins in commercial liquid saffron extracts (Saffr’activ®) were identified using high-performance chromatography (HPLC) with a diode array detector (DAD) and mass spectrometry (MS). The impact of storage on the qualities extract studied HPLC-DAD-MS by exposing trans-4-GG crocin to environmental factors. Light temperature induced degradation after only one week. Trans-4-GG was totally hydrolyzed when stored at 60 °C exposed light. A quick reliable method HPLC-DAD then developed improve quantification crocins extracts. An internal standard that uses response factor, corrected molecular weight each crocin, improved results for old